Qiagen gel extraction kit handbook

Rna extraction from plants using the rneasy plant mini kit. Dna fragments from 4 kb10 kb should be purified using the qiaquick gel extraction kit and dna fragments smaller than 70 bp or larger than 10 kb should be extracted with the qiaex ii gel extraction system specifications elution with just 10 l tris buffer or water highly concentrated purified dna dna fragments extracted in 18 minutes. Dna ranging from 70 bp to 10 kb is purified using a simple and fast bindwashelute procedure and an elution volume of 3050. In accordance with qiagens isocertified quality management system, each lot of the qiaamp dna mini kit and the qiaamp dna blood mini kit is tested against predetermined specifications to ensure consistent product quality. The innovative qiacube uses advanced technology to process qiagen spin columns, enabling seamless integration of automated, lowthroughput sample prep into your laboratory workflow. For cleanup of other enzymatic reactions, follow the protocol as described for pcr samples or use the minelute reaction cleanup kit. Dna ladder was run on a 2% agarose gel and 4 different fragment sizes 200 bp, 500 bp, 1 kb, and 5 kb were recovered using omega bioteks e. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. Minelute gel extraction kit 50 from qiagen selectscience.

Qiagen plasmid purification handbook 082003 5 kit contents qiagen plasmid kits qiagen plasmid kit midi 25 midi 100 maxi 10 maxi 25 maxi 100 catalog no. If purified dna is to be analyzed on a gel, add 1 volume of loading dye to 5 volumes of purified dna. The qiaamp dna investigator kit provides purification of genomic dna from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. The qiaquick pcr purification kit and qiaquick gel extraction kit can be fully automated on the qiacube. I wanted to know if i can use this kit to purify dna for further transfection in hek293 cells. Qiagen plasmid purification handbook 112005 3 contents kit contents 4 storage 5 quality control 5 product use limitations 6 product warranty and satisfaction guarantee 6 technical assistance 6 safety information 7 introduction 8 principle and procedure 8 equipment and reagents to be supplied by user 10 important notes 11 protocols plasmid or. Qiagen plasmid purification handbook 082003 3 contents kit contents 5 storage 8 technical assistance 8 product warranty and satisfaction guarantee 8 product use limitations 9 safety information 9 introduction 10 comparison of qiagen largescale plasmid purification options 10 principle and procedure. The innovative qiacube uses advanced technology to process qiagen spin columns, enabling seamless integration of automated, lowthroughput sample prep into. For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user manual. In this handbook, different protocols are provided for. Qiaquick spin handbook 072002 23 qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. Qiagen qiaquick gel extraction kit, 250 rxns, 30 to 50l elution volume, 10g binding capacity, dna sample, tube format, silica technology, manual processing, 70 bp to 10 kb fragment, fast and convenient procedure, for gel extractioncleanup of up to 10. Can qiaquick gel extraction kit purified dna be used for. The kit uses qiaamp minelute spin columns for purification of highquality dna with flexible elution volumes.

Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. This offer is valid until december 31, 2019 and only when order is placed online through qiagen web shop, your organizations purchasing system, your sales rep, or by email using the cyber19 promo code. Qiagen dneasy, for isolation of genomic dna from animal and tissues and cells, yeast, or bacteria. Qiaamp viral rna mini handbook 122007 5 product use limitations the qiaamp viral rna mini kit is intended for general laboratory use. For departmental related matters, please visit the department of molecular, cell, and developmental biology website.

All due care and attention should be exercised in the handling of the products. Product dneasy tissue kit 250 contents 250 dneasy spin columns. The qiaex ii gel extraction kit provides qiaex ii suspension together with binding and wash buffers, and a comprehensive handbook. Qiaquick spin handbook 032001 23 qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. The kit combines the selective binding properties of a silicabased membrane with flexible elution volumes of. Qiagens gel extraction kit is useful for extracting and purifying dna following gel confirmation band size of our pcr products of interest. In the qiagen gel extraction kit, it says to dissolve at 50c or until completely dissolved. For more information, please refer to the qiaquick spin handbook. The minelute qiaquick gel extraction kit provides spin columns for gel extraction. Forceps are to be sterilized in fine science tools heat block at. Qiagen qiaquick gel extraction kit 28704 and 28706. Gel extraction kit and a comparable kit from company q following manufacturers recommended protocols.

The kit combines the selective binding properties of a silicabased membrane with flexible elution volumes of between 20 and 100 l. Apr 08, 2008 if you use qiagens gel extraction kit, you may have noticed that after each spin, a small droplet tends to form inside the column at the side that was to the outside of the centrifuge rotor due to the shape of the column. Jul 20, 2015 this tutorial explains how to carry out a miniprep using the qiaprep spin miniprep kit provided by qiagen. Banerjee lab ucla molecular, cell and developmental biology. For departmental related matters, please visit the department of.

Up to 400 mg agarose can be processed per spin column. Its probably not too important during most of the procedure, but its worth bearing in mind at the elution step. Can qiaquick gel extraction kit purified dna be used for cell transfection. Protocol qiaquick spin handbook 032001 23 qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp qiaquick gel extraction kit for gel the same steps as the manual qg and leads to inefficient dna binding. Qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. If you use qiagens gel extraction kit, you may have noticed that after each spin, a small droplet tends to form inside the column at the side that was to the outside of the centrifuge rotor due to the shape of the column. The minelute gel extraction kit removes nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from dna samples, delivering highly concentrated dna suitable for a variety of downstream applications see figure higher dna concentrations. Mix the solution by pipetting up and down before loading the gel. For more information, please refer to the qiaquick spin handbook, march 2008. Qiavac vacuum manifolds 30 handling guidelines for qiavac 24 31 qiavac 6 32 ordering information 33 qiagen companies and distributors 35 qiaquick spin handbook 032001 3. For purification of up to 100 g total rna from cells, tissues, and yeast. Isolate a suitable piece of tissue and place in a uvcrosslinked 1. Electrophoretic analysis of recovered dna from an agarose gel compared to the control. Qiavac vacuum manifolds 30 handling guidelines for qiavac 6 31 qiavac 24 32 ordering information 33 qiagen companies and distributors 35 qiaquick spin handbook 072002 3.

Qiagen research kits compatible with the qiavac 24 plus application kit accessories plasmid minipreps qiaprep spin miniprep kits dna cleanup qiaquick pcr purification kits qiaquick gel extraction kits qiaquick nucleotide removal kits minelute pcr purification kits. Qiagen plasmid purification handbook harvard university. Qiaquick gel extraction kit 250 from qiagen selectscience. Protocols are provided for purification of dna from agarose gels, solutions, and polyacrylamide gels. Qiagen kit for dna extraction from agarose gels biocompare.

Qiaquick gel extraction kit protocol using a microcentrifuge. Qiaquick gel extraction kit protocol syracuse university. Rneasy mini handbook rneasy mini kit for purification of total rna from animal cells, animal tissues, bacteria, and yeast, and for rna cleanup rneasy protect mini kit for immediate stabilization of rna in harvested animal tissues and subsequent total rna purification rneasy plant mini kit for purification of total rna from plants and filamentous fungi fourth edition june. Mar 01, 2017 qiagen s gel extraction kit is useful for extracting and purifying dna following gel confirmation band size of our pcr products of interest. Lyophilized qiagen protease can be stored at room temperature 1525c for up to. We have used this kit to extract many pcr products of various sizes up to 10 kb, and the simple procedure consistently provides us with intact, pure products for downstream analysis.